Coffee is one of the most commonly consumed drinks in the world, and it has been shown to have health benefits. Researchers from India evaluated the anticancer properties of chlorogenic acid complex, which is an active compound in green coffee beans. Their findings appeared in the Food Science and Human Wellness journal.
Chlorogenic acid (CGA) is the main ingredient in coffee beans. It has been studied for its therapeutic effects, but there are limited studies that have investigated its anticancer properties.
The researchers of the present study used chlorogenic acid complex (CGA7), a standardized decaffeinated water-soluble green coffee bean extract and evaluated its cytotoxic effect on human and mouse cancer cell lines.
The researchers cultured human cervical (HeLa), liver (HepG2), breast (MCF7), colon cancer (HCT-15, HCT-116), cell lines and mouse B16-F1, EAC, normal cell line MEF (STO).
The researchers found that CGA7 treatment induces dose- and time-dependent apoptosis or cell death in different cancer cell lines.
The CGA7-induced apoptosis was characterized by DNA fragmentation, PARP-1 cleavage, caspase-9 activation, and downregulation of Bcl-2 -- an anti-apoptotic protein -- and upregulation of pro-apoptotic protein BAX.
The findings indicated that CGA7 in green coffee beans is a potent anticancer compound that could be a safe bioactive ingredient for the prevention of cancer.
Gouthamchandra K, Sudeep H, Venkatesh B, Prasad KS. CHLOROGENIC ACID COMPLEX (CGA7), STANDARDIZED EXTRACT FROM GREEN COFFEE BEANS EXERTS ANTICANCER EFFECTS AGAINST CULTURED HUMAN COLON CANCER HCT-116 CELLS. Food Science and Human Wellness. 2017;6(3):147–153. DOI: 10.1016/j.fshw.2017.06.001