In this study, researchers from Fayoum University and Cairo University in Egypt screened the anti-microbial activities of some plant essential oils against isolates of Candida albicans, which causes vulvovaginal candidiasis (VVC), and investigated the anti-fungal effect of the most active essential oil against planktonic and sessile cells. Their findings were published in the Journal of Herbal Medicine.
To screen the biofilm production of C. albicans, the researchers used tissue culture plate method.
They tested the fungus' susceptibility to the anti-fungal fluconazole using disk diffusion method.
To screen the anti-fungal activities of nine essential oils, the researchers used strong biofilm-producer, fluconazole-resistant or dose-dependently susceptible clinical isolates of C. albicans ATCC 10231.
They then performed microdilution assay to determine the minimum inhibitory concentration (MIC) of fennel essential oil, fluconazole and their combination against planktonic and sessile cells.
The researchers reported that 10 out of 19 C. albicans strains showed strong ability to form biofilms.
None of the tested clinical isolates was sensitive to fluconazole.